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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene continues to be observed in several tumors, which ends up in chromosome instability, and so could add to tumorigenesis. This protein was found to associate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a important function in promoting cell-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, have already been explained. Two supplemental splice variants were noted but thorough nucleotide sequence information and facts is just not yet obtainable.

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Blocking peptides are peptides that bind exclusively on the focus on antibody and block antibody binding. These peptide typically contains the epitope acknowledged via the antibody.

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This guidebook click here outlines further more feasible optimization of cellular and immunoassay parameters to make sure the best possible effects are...

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This guidebook outlines further more feasible optimization of cellular and immunoassay parameters to be certain the absolute best benefits are...

Antibodies sure to the blocking peptide now not bind towards the epitope within the goal protein. By evaluating the staining through the blocked antibody as opposed to the antibody by yourself, you can see which staining is certain.

Goal Details Cyclin E belongs for the extremely conserved cyclin family, whose users are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins show unique expression and degradation patterns which lead for the temporal coordination of each mitotic celebration. Cyclin E kinds a complex with and capabilities to be a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates with the G1-S stage boundary and is degraded as cells progress as a result of S stage.

The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by the two antibodies...

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